Polyclonal Treg were isolated from total nucleated cells obtained through leukapheresis of healthy donors by CD8 + cell depletion and subsequent CD25 high enrichment.
#TEXMACS T CELL ISOLATION MANUAL#
Here we show results from our approach to translate manual Treg manufacturing to the fully closed automated CliniMACS Prodigy ® system reducing contamination risk, hands-on time, and quality variation from human intervention. For the majority of applications, clinical administration of Treg requires laborious ex vivo expansion and typically involves open handling for culture feeds and repetitive sampling. 5National Center for Tumor Diseases, Dresden, GermanyĪdoptive transfer of T regulatory cells (Treg) has been successfully exploited in the context of graft-versus-host disease, transplantation, and autoimmune disease.4DFG-Center for Regenerative Therapies Dresden, Center for Molecular and Cellular Bioengeneering, Technische Universität Dresden, Dresden, Germany.3Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.2Department of Hematology, Medical Clinic I, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.1GMP Facility, DFG-Center for Regenerative Therapies Dresden, Center for Molecular and Cellular Bioengeneering, Technische Universität Dresden, Dresden, Germany.José Manuel Marín Morales 1, Nadine Münch 1, Katja Peter 1, Daniel Freund 1, Uta Oelschlägel 2, Kristina Hölig 2, Thea Böhm 3, Anne-Christine Flach 3, Jörg Keßler 3, Ezio Bonifacio 4, Martin Bornhäuser 2,5 and Anke Fuchs 1,2,4 *
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